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Image Search Results
Journal: Oxidative medicine and cellular longevity
Article Title: Inhibition of α -Synuclein Accumulation Improves Neuronal Apoptosis and Delayed Postoperative Cognitive Recovery in Aged Mice.
doi: 10.1155/2021/5572899
Figure Lengend Snippet: Figure 3: Peripheral surgery increased mitochondrial α-syn accumulation and damage. (a) The hippocampus was stained to determine colocalization of α-syn (red) and COXIV (an inner mitochondrial membrane (green)). Cell nuclei were stained with DAPI (blue). Arrows point to mitochondrial α-syn; scale bars = 10 μm, n = 5. (b) Transmission electron microscopy showing mitochondrial morphology in the hippocampus after surgery (n = 3 mice per group) and assessment of abnormal cristae (n = 57 −60 cells). Scale bars = 500 nm. Results are presented as mean ± SEM (one-way analysis of variance). ∗∗∗P < 0:001, control (Con) or anesthesia-alone (Ane) vs. surgery plus anesthesia (Sur).
Article Snippet: Accumulation of SNCA oligomers was detected by immunohistochemistry using a rabbit polyclonal SNCA oligomer-specific Syn33 antibody (ABN2265; Merck Millipore, Billerica, MA, USA), a mouse monoclonal antibody against total SNCA (4D6, ab1903; Abcam), a
Techniques: Staining, Membrane, Transmission Assay, Electron Microscopy, Control